Capillary Electrophoresis (CE) is an electrophoretic technique in which separation of molecules is achieved by their different electrophoretic mobility in a narrow-bore capillary tube filled with an electrolyte. CE is a powerful separation technique that has been used for a variety of applications, including DNA sequencing, protein analysis, and药物 discovery. It is faster and uses less sample than many other electrophoretic techniques.
CE is based on the principle of electrophoresis, which is the migration of charged molecules through an electric field. In CE, a voltage is applied across a capillary tube filled with an electrolyte to create an electric field. The sample is injected into the capillary tube, and the molecules in the sample migrate through the electric field. The rate of migration depends on the net charge of the molecule, the size of the molecule and the strength of the electric field. Smaller molecules migrate faster than larger molecules and highly charged molecules migrate faster than molecules with a lower charge.
CE has been used for a wide variety of applications, including:
CE has several advantages over other electrophoretic techniques, including: